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plus reagent (lipofectamine ltx kit)  (Thermo Fisher)


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    Thermo Fisher plus reagent (lipofectamine ltx kit)
    Plus Reagent (Lipofectamine Ltx Kit), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plus+reagent+%28lipofectamine+ltx+kit%29/pmc12219648-327-28-43?v=Thermo+Fisher
    Average 90 stars, based on 1 article reviews
    plus reagent (lipofectamine ltx kit) - by Bioz Stars, 2026-07
    90/100 stars

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    Misfolded Httex1 causes nuclear depletion and sequestration of endogenous TDP43 to Httex1 aggregates. Representative images of HEK293T cells 24 h after <t>transfection</t> with Httex1(Q72) expression construct. Httex1 is visualized by PHP1 antibody ( magenta ), endogenous TDP43 is visualized by TDP43 antibody ( green ) and cell nuclei are visualized by DAPI counterstain ( blue ). The arrows highlight cells with cytoplasmic Httex1(Q72) and normal nuclear TDP43 labeling. Arrowheads point to cells with aggregates where Httex1(Q72) and TDP43 colocalize and TDP43 signal appears depleted from the nucleus. Scale bar is 20 μm for all the panels.
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    Misfolded Httex1 causes nuclear depletion and sequestration of endogenous TDP43 to Httex1 aggregates. Representative images of HEK293T cells 24 h after <t>transfection</t> with Httex1(Q72) expression construct. Httex1 is visualized by PHP1 antibody ( magenta ), endogenous TDP43 is visualized by TDP43 antibody ( green ) and cell nuclei are visualized by DAPI counterstain ( blue ). The arrows highlight cells with cytoplasmic Httex1(Q72) and normal nuclear TDP43 labeling. Arrowheads point to cells with aggregates where Httex1(Q72) and TDP43 colocalize and TDP43 signal appears depleted from the nucleus. Scale bar is 20 μm for all the panels.
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    Misfolded Httex1 causes nuclear depletion and sequestration of endogenous TDP43 to Httex1 aggregates. Representative images of HEK293T cells 24 h after <t>transfection</t> with Httex1(Q72) expression construct. Httex1 is visualized by PHP1 antibody ( magenta ), endogenous TDP43 is visualized by TDP43 antibody ( green ) and cell nuclei are visualized by DAPI counterstain ( blue ). The arrows highlight cells with cytoplasmic Httex1(Q72) and normal nuclear TDP43 labeling. Arrowheads point to cells with aggregates where Httex1(Q72) and TDP43 colocalize and TDP43 signal appears depleted from the nucleus. Scale bar is 20 μm for all the panels.
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    Image Search Results


    Misfolded Httex1 causes nuclear depletion and sequestration of endogenous TDP43 to Httex1 aggregates. Representative images of HEK293T cells 24 h after transfection with Httex1(Q72) expression construct. Httex1 is visualized by PHP1 antibody ( magenta ), endogenous TDP43 is visualized by TDP43 antibody ( green ) and cell nuclei are visualized by DAPI counterstain ( blue ). The arrows highlight cells with cytoplasmic Httex1(Q72) and normal nuclear TDP43 labeling. Arrowheads point to cells with aggregates where Httex1(Q72) and TDP43 colocalize and TDP43 signal appears depleted from the nucleus. Scale bar is 20 μm for all the panels.

    Journal: The Journal of Biological Chemistry

    Article Title: TDP43 and huntingtin Exon-1 undergo a conformationally specific interaction that strongly alters the fibril formation of both proteins

    doi: 10.1016/j.jbc.2024.107660

    Figure Lengend Snippet: Misfolded Httex1 causes nuclear depletion and sequestration of endogenous TDP43 to Httex1 aggregates. Representative images of HEK293T cells 24 h after transfection with Httex1(Q72) expression construct. Httex1 is visualized by PHP1 antibody ( magenta ), endogenous TDP43 is visualized by TDP43 antibody ( green ) and cell nuclei are visualized by DAPI counterstain ( blue ). The arrows highlight cells with cytoplasmic Httex1(Q72) and normal nuclear TDP43 labeling. Arrowheads point to cells with aggregates where Httex1(Q72) and TDP43 colocalize and TDP43 signal appears depleted from the nucleus. Scale bar is 20 μm for all the panels.

    Article Snippet: Cells were transfected with 500 ng of Httex1(Q72) plasmid DNA (Genscript) using Lipofectamine LTX with Plus Reagent transfection kit (ThermoFisher), according to the manufacturer’s protocol.

    Techniques: Transfection, Expressing, Construct, Labeling